Comparative cryopreservation of bovine and porcine primary hepatocytes

The isolation of primary hepatocytes from liver tissue farm animals yields a very high number cells, and part them can be stored by cryopreservation for future experiments. As no experience exists with the cattle, our study aimed at bovine use different protocols compared pig. We tested freezing media (William’s Medium E vs. University Wisconsin solution), cryoprotectants (dimethyl sulfoxide without trehalose as additional additive), systems (standard container controlled-rate freezer) times (4 28 d). These tests identified general influence species systems, whereas media, additive time was less or not obvious. In this regard, we determined mean recovery 30% 55% porcine cryopreserved in freezer, rates were about 10% when frozen standard container. accordance observation, cultivation cattle effective than that hepatocytes. Hepatocytes successfully partially cultured after but lower percentage